Part:BBa_K4907036
gfp
Biology
The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence. Its excitation wavelength is 488 nm, and its emission wavelength is 533 nm. When gfp is expressed in bacteria, it produces non-exocytosed GFP proteins that cause the bacteria to fluoresce green at an excitation wavelength of 488 nm. It is a commonly used reporter for expressing and tracing.
Usage and Design
To better monitor the growth status of the bacteria and to test the performance of our constructed promoters, GFP was introduced as a reporter. We can measure the time curve to judge its dynamic performance or record the fluorescence intensity for comparison of promoters with different strengths.
This gfp sequence is used in many sections of our project, such as building the composite part: BBa_K4907146, BBa_K4907100, BBa_K4907101, BBa_K4907109, etc. All of them use this gfp sequence in different application scenarios.
Please click the composite part directly for more detailed information.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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